CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.

ELsobky, Muhammad; Abdel-lateif, Khalid Salah; Fahmi, Abdelmegid; Elzanaty, Abdelfatah; Eissa, Ragaa

doi:10.21608/mjab.2024.271332.1016

CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.

Document Type : original papers

Authors

¹ Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt

² Department of Genetics, Faculty of Agriculture, Menoufia University

³ Genetics Department Faculty of agriculture Menoufia Univesity Shibin El kom

10.21608/mjab.2024.271332.1016

Abstract

: Twenty-six isolates used in qualitative screening of Cellulase done in two media CMC-Congo red dye plate and 1% PASC (phosphoric acid swollen cellulose) plate. Quantitative Enzymes activities were tested in two fermentation methods SSF (solid state fermentation) and SmF (Submerged fermentation). SmF was detected at 2,3,4,6 and 7 days in Mandel’s Media with CMC 0.5%. Moreover, SSF was carried out by using straws (Rice and wheat) for all isolates. The cellulase activities measured as (FPase, CMCase and β-glucosidase). In this study positive correlation coefficient in enzymes activity between SSF and qualitative screening in (CMC & PASC) plate. Higher correlation have been detected between PACS and SSF rice straw was about (0.595**) for β-Glucosidase. After all these tests we can compare enzyme production by SmF and SSF. Data showed SmF production for high yield and high quality. The cellulase genes, Chb gene encoding cellobiohydrolase 2 (CBH2) and Bgl gene that encoding endo-β-gluconase (BGL1) were present in all Trichoderma isolates.

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