ELsobky, M., Abdel-lateif, K., Fahmi, A., Elzanaty, A., Eissa, R. (2024). CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.. Menoufia Journal of Agricultural Biotechnology, 9(2), 23-38. doi: 10.21608/mjab.2024.271332.1016
Muhammad ELsobky; Khalid Salah Abdel-lateif; Abdelmegid Fahmi; Abdelfatah Elzanaty; Ragaa Eissa. "CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.". Menoufia Journal of Agricultural Biotechnology, 9, 2, 2024, 23-38. doi: 10.21608/mjab.2024.271332.1016
ELsobky, M., Abdel-lateif, K., Fahmi, A., Elzanaty, A., Eissa, R. (2024). 'CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.', Menoufia Journal of Agricultural Biotechnology, 9(2), pp. 23-38. doi: 10.21608/mjab.2024.271332.1016
ELsobky, M., Abdel-lateif, K., Fahmi, A., Elzanaty, A., Eissa, R. CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.. Menoufia Journal of Agricultural Biotechnology, 2024; 9(2): 23-38. doi: 10.21608/mjab.2024.271332.1016
CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.
1Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt
2Department of Genetics, Faculty of Agriculture, Menoufia University
3Genetics Department Faculty of agriculture Menoufia Univesity Shibin El kom
Abstract
: Twenty-six isolates used in qualitative screening of Cellulase done in two media CMC-Congo red dye plate and 1% PASC (phosphoric acid swollen cellulose) plate. Quantitative Enzymes activities were tested in two fermentation methods SSF (solid state fermentation) and SmF (Submerged fermentation). SmF was detected at 2,3,4,6 and 7 days in Mandel’s Media with CMC 0.5%. Moreover, SSF was carried out by using straws (Rice and wheat) for all isolates. The cellulase activities measured as (FPase, CMCase and β-glucosidase). In this study positive correlation coefficient in enzymes activity between SSF and qualitative screening in (CMC & PASC) plate. Higher correlation have been detected between PACS and SSF rice straw was about (0.595**) for β-Glucosidase. After all these tests we can compare enzyme production by SmF and SSF. Data showed SmF production for high yield and high quality. The cellulase genes, Chb gene encoding cellobiohydrolase 2 (CBH2) and Bgl gene that encoding endo-β-gluconase (BGL1) were present in all Trichoderma isolates.
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