PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM

Dwedar, Shimaa; Hamid, Marwa S. Abdel-; Keshk, Doaa; Haroun, A.A.; El Baz, A.F.

doi:10.21608/mjab.2015.322270

PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM

Document Type : original papers

Authors

¹ (1) Industerial Biotechnology Dept., Genetic Engineering and Biotechnology Institute, Sadat city

² ) Microbial Biotechnology Dept.2, Genetic Engineering and Biotechnology Institute, Sadat city university, Egypt

³ (3) Genetic Engineering and Biotechnology Institute , Agriculture Research Center, Giza- Egypt

⁴ (4) Division of Chemical Industries Research, National Research Center, Dokki, Egypt.

10.21608/mjab.2015.322270

Abstract

ABSTRACT: Siderophores are ferric ion specific chelating agents produced by bacteria and fungi growing under low iron stress. The role of these compounds is to scavenge iron from the environment and to make the mineral, which is an essential metal, available to the microbial cells. Azotobacter sp. isolate Azo-4 isolated from Sadat City ,Egypt showed positive result for siderophores production by Chrome Azurol Sulfonate (CAS) assay. The organism was subjected to various biochemical tests and 16S rRNA analysis; results indicated that the isolated fragment sequence (~1.3) Kbp is homologous to Azotobacter chroococcum strain KCA1 16S ribosomal RNA gene. Deferrated medium concentration induced the siderophores production (45 µM). Hydroxymate siderphore was extracted and the antagonistic activity of partially purified siderphore was tested against bacterial pathogens Bacillus stbtlius and Salmonella sp.,in vitro. Result showed that the Azotobacter sp. under study is a good producer of siderophores, which can be beneficial for its antagonistic activity towards different pathogens as biocontrol agent.

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