BIOCHEMICAL AND PATHOLOGICAL CHARACTERIZATION OF LEAF RUST RESISTANCE IN SOME EGYPTIAN WHEAT CULTIVARS

. Elsawy, Marihan F; . El-Naggar, ), Doaa R; . Elabsawy, S. A; Yassen, Y. M.; Abdellatif, K. F.

doi:10.21608/mjab.2015.321114

BIOCHEMICAL AND PATHOLOGICAL CHARACTERIZATION OF LEAF RUST RESISTANCE IN SOME EGYPTIAN WHEAT CULTIVARS

Document Type : original papers

Authors

¹ (1) Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Minoufiya, Egypt.

² (2) Wheat Disease Department, Plant Pathology Research Institute, Agricultural Research Center, Egypt.

³ (3) Bioinformatics Department, GEBRI, University of Sadat City, Sadat City, Minoufiya, Egypt.

10.21608/mjab.2015.321114

Abstract

ABSTRACT: Leaf rust of wheat is caused by the fungus Puccinia triticina Eriks. (syn. P. recondita Rob. Ex Desm. f. sp. tritici Eriks. And Henn.). Six Egyptian wheat cultivars (Triticum aestivum L.) (i.e. Giza139, Giza168, Gemmiza7, Gemmiza9, Misr1 and Sakha93) have been used to study pathological and biochemical characterization of leaf rust resistance. The tested six wheat cultivars were screened for seedling reaction and adult plant response against leaf rust pure race (PTSS), under greenhouse conditions. This screening was carried out concerning the infection type, final rust severity and AUDPC. Results indicated that Misr-1 was completely resistant, at both stages. Giza-168 and Gemmiza-9 wheat cultivars recorded low values of the estimated parameters in adult plant reaction. Meanwhile, Giza-139 and Gemmiza-7 were susceptible where they recorded high values of the estimated parameters. Some protein bands are correlated with rust resistance in wheat at molecular weight at about 17 kDa and 12 kDa (for total protein pattern) and at 140 kDa and 34 kDa (for the water soluble protein pattern) mainly for “Misr1” wheat cultivar (completely rust resistance cultivar according to the pathological experiment). Thus it can be said that biochemical and pathological experiments revealed that Misr-1 wheat cultivar is rust resistant. It can be also said that biochemical markers could be used to screen whether a wheat cultivar is rust resistant or not because some protein bands have been identified to be correlated with rust resistace. Thus biochemical markers can be used to save our effort and time to determine the rust resistant and susceptible wheat cultivars.

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