DIVERSITY OF TEN RHIZOBIUM LEGUMINOSARUM ISOLATED FROM VARIOUS EGYPTIAN SOIL ON PHENOTYPIC BASIS

	DIVERSITY OF TEN RHIZOBIUM LEGUMINOSARUM ISOLATED FROM VARIOUS EGYPTIAN SOIL ON PHENOTYPIC BASIS
Article 1, Volume 40, Issue 1, February 2015, Page 57-65 PDF (908.86 K)
Document Type: original papers
DOI: 10.21608/mjab.2015.321102
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Authors
Enas. A . Mahrous; Ragaa A. Eissa; H.H. Nagaty; A.M. El Zanaty
Genetics Dep. Faculty of Agriculure-Menofia University .
Abstract
ABSTRACT: We used ten Rhizobium leguminosarum isolates that obtained from different Egyptian soils with different pH . The isolates were characterized based on phenotypic traits , Temp, pH and their response to different antibiotics resistance. All isolates grew well at temperatures of 28,30 and 35 °C not able to grow at 45°C . Four isolates showed growth inhibition at 40°C . The isolates showed variation in growth at various pH levels in their medium at 4, 5, 9,10 and 11. Isolate number 9 showed the ability to grow at all used pH levels. Isolates number 4 and 8 showed inability to grow at all tested pH levels. We investigated the effects of various antibiotics including S10, MEM10, Cxm30, Va30, Ox1, T30, N30, B, Am10 and k30 on growth of Rhizobium isolates . We found all isolates resistant to antibiotic B and all isolates were sensitive to antibiotic k30.
Keywords
Rhizobium leguminosarum; temperature; pH and antibiotic biodiversity. 57


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