Hussain, K., Draz, S., Khalil, S., Ebrahiem, A. (2017). ESTIMATION OF TOTAL PHENOLIC, FLAVONOID CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFERA VARIETY GROWN IN EGYPT. Menoufia Journal of Agricultural Biotechnology, 2(2), 15-24. doi: 10.21608/mjab.2017.176211
K. E. Hussain; Sh. N. Draz; Samia M. Khalil; A.H.A. Ebrahiem. "ESTIMATION OF TOTAL PHENOLIC, FLAVONOID CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFERA VARIETY GROWN IN EGYPT". Menoufia Journal of Agricultural Biotechnology, 2, 2, 2017, 15-24. doi: 10.21608/mjab.2017.176211
Hussain, K., Draz, S., Khalil, S., Ebrahiem, A. (2017). 'ESTIMATION OF TOTAL PHENOLIC, FLAVONOID CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFERA VARIETY GROWN IN EGYPT', Menoufia Journal of Agricultural Biotechnology, 2(2), pp. 15-24. doi: 10.21608/mjab.2017.176211
Hussain, K., Draz, S., Khalil, S., Ebrahiem, A. ESTIMATION OF TOTAL PHENOLIC, FLAVONOID CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFERA VARIETY GROWN IN EGYPT. Menoufia Journal of Agricultural Biotechnology, 2017; 2(2): 15-24. doi: 10.21608/mjab.2017.176211
ESTIMATION OF TOTAL PHENOLIC, FLAVONOID CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFERA VARIETY GROWN IN EGYPT
1Department of Biochemistry, Faculty of Agriculture, University of Menoufia, Egypt
2Kaha Central Hospital
Abstract
The present research determined the chemical composition and the antioxidant activity of leaves extracts (aqueous – acetone) of Moringa oleifera variety grown in Egypt. It was found that moringa leaves contain total ash 8.03%, crude lipids 3.14%, crude protein 26.31%, total carbohydrates 44.89%, and crude fiber 17.63%. Total phenolics in leaves aqueous extract were 243.8 mg/g and total flavonoids were 145.22 mg/g, while in acetone extract total phenolics were 142.51 mg/g and total flavonoids were 124.64 mg/g. HPLC results showed that moringa leaves contained 23 phenolic compounds and 22 flavonoid contents and the main constituents of both were (e-vanillic, Pyrogallol, Salycilic, Catechin, P-hydroxybenzoic, Ellagic, Alpha-coumaric, 3,4,5-trimethoxy-cinnamic, Protocatechuic and Chlorogenic) and (Luteo-6-arabinose-8-gloucose, Hesperidin and Rosmarinic) respectively. In comparison with the acetone extract, the aqueous extract showed the highest activity in reducing power assay. Egyptian moringa leaves extracts were found to have antioxidant activity due to their high amount of total phenolics and falvonoids.
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