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Hammam, M., Khalil, G., ElGawad, S., Bisi, M., Mohamed, I. (2020). HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS). Menoufia Journal of Agricultural Biotechnology, 5(1), 11-22. doi: 10.21608/mjab.2020.169570
M. A. Hammam; G. A. Khalil; S. M. Abd ElGawad; Manal K. I. El- Bisi; I. A. A. Mohamed. "HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS)". Menoufia Journal of Agricultural Biotechnology, 5, 1, 2020, 11-22. doi: 10.21608/mjab.2020.169570
Hammam, M., Khalil, G., ElGawad, S., Bisi, M., Mohamed, I. (2020). 'HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS)', Menoufia Journal of Agricultural Biotechnology, 5(1), pp. 11-22. doi: 10.21608/mjab.2020.169570
Hammam, M., Khalil, G., ElGawad, S., Bisi, M., Mohamed, I. HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS). Menoufia Journal of Agricultural Biotechnology, 2020; 5(1): 11-22. doi: 10.21608/mjab.2020.169570

HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS)

Article 3, Volume 5, Issue 1, January and February 2020, Page 11-22  XML PDF (1.03 MB)
Document Type: original papers
DOI: 10.21608/mjab.2020.169570
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Authors
M. A. Hammam1; G. A. Khalil1; S. M. Abd ElGawad1; Manal K. I. El- Bisi2; I. A. A. Mohamed3
1Department of Biochemistry Faculty of Agricultural Menofia Univeristy shibin El-Kom, Egypt
2Prof. Doctor at chemical and technology of textile National research center
3Ministry of Health and Population, Egypt.
Abstract
Green synthesis of silver nanoparticles (AgNPs) is non-toxic, rabid and eco-friendly than commonly used physicochemical methods. This study focuses on synthesis, characterization, hepatoprotective effect and antioxidant activity of AgNPs synthesizes from Cymbopogan Citratus (Lemongrass). Silver nanoparticles were formed within 30 minutes by heating to 80oC magnetic stirrer using aqueous solution of AgNO3 (0.1 N) with fresh leaves methanolic extract of Cymbopogan Citratus. The synthesized silver nanoparticles were characterized by using UV-visible spectrophotometer analysis and transmission electron microscope (TEM). The antioxidant activity of nanoparticles were determined in vitro by reducing power, total antioxidant capacity and free radical scavenging activity by (DPPH). and experimental study was designed to evaluate the antioxidant activity and hepatoprotective effect of silver nanoparticeles, methanolic extract and essential oil comparing with vitamin E.
Results: (UV-Vis) spectrum of synthesized AgNPs shows a peak at 412 nm and the size of silver nanaoparticels was 13.5 nm by (TEM).
Silver nanoparticles showed potent hepatoprotective effect and antioxidant activity in both in vitro and in vivo methods.  
Keywords
Silver nanoparticles (AgNPs); green synthesis; lemon grass; antioxidant activity; DPPH (2; 2-diphenyl -1- pricrylhydrazyl)
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