ORIGINAL_ARTICLE
EFFECT OF AQUEOUS PSIDIUM GUAJAVA LEAVES EXTRACT AS ANTI - HYPERGLYCEMIC AGENT
Background: Hyperglycemia is one of the serious problems that affect humans' lifestyle and require special treatments. Incorrect use of synthetic drugs results in side effects and other problems. Diabetic patients need safer and more effective anti-diabetic drugs that decrease diabetic complications. Natural products are harmless due to conventional medicine can be inefficient (e.g. side effects and ineffective therapy).
Objective: Evaluate anti- hyperglycemic activity of aqueous leaves extract of Psidium guajava in hyperglycemic rats.
Methods: Adult males albino rats, weighting (140-150 grams) were assigned to one of four groups of six animals each (n= 6). Hyperglycemia induced by single dose intraperitoneal injection of STZ - NA (60 mg/kg - 110 mg/kg, b.wt i.p.), the control groups was given distilled water, the experimental sub-groups were started one week later of STZ induction, then treated once daily by aqueous extract of Psidium guajava leaves until the end of experiment. Initially, blood samples were taken for measured glucose levels and HbA1c. The body weight, urine sugar and blood glucose levels were checked regularly.
Results: The polyphenolic of guava leaves extract was found to be (227.63 mg GAE /g. Ex), while flavonoids content was (79.96 mg QE/g. Ex). However, it caused a significant decrease in blood glucose levels and HbA1c and other glycemic indicators.
Conclusion: aqueous extract of Psidium guajava leaves possess significant anti-diabetic, and effective in lowering blood glucose level but lack hypoglycemic effect in hyperglycemic rats. Further studies warranted to elucidate possible mechanism(s) of action of the plant material.
https://mjab.journals.ekb.eg/article_207622_c53a29aa7335c38fff1e28099738a1b3.pdf
2021-11-01
83
93
10.21608/mjab.2021.207622
hyperglycemia
Hypoglycemia
anti-hyperglycemic
diabetic
natural products
Psidium guajava
Streptozotocin and Rats
Omima M. R.
Abu Elez
1
Pathological of Biochemistry Dept. - Medical Analysis- El-Roda Hospital- Damietta - Egypt
AUTHOR
M. A.
Hammam
2
Biochemistry Department- Faculty of Agricultural- Menuofia University-Egypt
AUTHOR
F.M.
Elshony
3
Biochemistry Department- Faculty of Agricultural- Menuofia University-Egypt
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF SPRAYING BLUE GREEN ALGAE (CYANOBACTERIA) EXTRACTS ON HYBRID RICE SEED PRODUCTION
Two isolates of cyanobacteria (Anabaena oryzae, Nosto centophytum) were isolated from agricultural wastewater from the experimental farm of Sakha Research Station, Sakha, Kafr EL Sheikh, Egypt for testing their ability to fix atmospheric nitrogen and produce hormones and its effect on hybrid rice seed production by spraying the isolates extract and their mixture on the rice plants. Isolates were varied in their capacity in the extracellular-nitrogen, Intracellular secreted, produce hormones and dry weight of the cyanobacteria isolates (mg/100 ml-culture) where the lowest content was for isolate Anabaena oryzae and the highest was for Nostoc centophytum and mixture. The aim of the present investigation is to identify the effect of cyanobacteria extract isolated from Egyptian waste water on hybrid rice seed production. The used cytoplasmic male sterile lines were IR69625A and G46A and the restorer line was G.178. Application of mixture between Anabaena oryzae and Nostoc centophytum produced the highest values in most studied characters such as seed yield in both seasons.
https://mjab.journals.ekb.eg/article_207623_24faf37a437cc9a3ce0215c394f2dd5a.pdf
2021-11-01
71
81
10.21608/mjab.2021.207623
Hybrid Rice
CMS
Cyanobacteria
Anabaena oryzae
Nostoc centophytum
seed yield
H. A. H.
EL-Zawawy
1
Botany Dept. (Microbiology& General Botany) Fac. of Agric. Al-Azhar Univ. Cairo,, Egypt.
AUTHOR
E.A.E.
Abd El-aziz
2
Botany Dept. (Microbiology& General Botany) Fac. of Agric. Al-Azhar Univ. Cairo,, Egypt.
AUTHOR
H. Sh.
Hamad
3
Rice Research Department, Field Crops Research Institute, Agriculture Research Centre, Sakha, Kafrelsheikh, Egypt.
AUTHOR
E. F.
Arafat
4
Rice Research Department, Field Crops Research Institute, Agriculture Research Centre, Sakha, Kafrelsheikh, Egypt.
AUTHOR
ORIGINAL_ARTICLE
HEPATOPROTECTIVE EFFECTS OF TURMERIC AND MILK THISTLE SEED FLOUR AGAINST ETHANOL LIVER DAMAGE IN WISTER RATS
Chemical composition of milk thistle seed flour )MTSF) and turmeric (T) were investigated, for milk thistle seed flour, moisture (8.0%), protein (13.0 %), carbohydrates (27.55%), crude lipid (15.0%), ash (10.45%), and crude fiber (26.0%), while turmeric has moisture (8.1%) , protein (9.3 %), carbohydrates (57%), crude lipid (17.0%), ash (6.0%) and crude fiber (2.6%). For total phenol, the highest value in turmeric (11.57mg GAE/g) while milk thistle seed flour (3.39 mg GAE/g)) also total flavonoid for turmeric (4.9mg) while and in milk thistle seed flour (2.34mg). DPPH for milk thistle recorded the highest percent of (FRSA) 98.5 % and turmeric recorded lowest present 82.2% in concentration 100 µg/ml. Reducing power for milk thistle recorded 0.651 but turmeric (T) recorded 0.502 in concentration 100 µg/ml . The identification of phenolic compounds for plant extracts were investigated by HPLC ,the highest content in milk thistle seed flour (MTSF) was Benzoic acid (103.33 ppm) while the lowest content was Caffeine(1.07 ppm) but in turmeric(T) the highest content was Benzoic acid (208.41 ppm), while the lowest content was quercitin (3.08 ppm).The level of plasma aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , alkaline phosphatase (ALP) , total bilirubin , total protein and albumin and plasma antioxidant state SOD , CAT, GPx and MDA were determined to assay hepatotoxicity.
https://mjab.journals.ekb.eg/article_207624_76057b00d46b6929ad751627b4bf5477.pdf
2021-11-01
57
69
10.21608/mjab.2021.207624
Milk thistle seed flour
disorder
Biochemical analyss
F. M.
El-Shouny
1
Biochemistry- Dep., Faculty of Agricultural, Menoufia University
AUTHOR
S. K.
El-Kadousy
2
Biochemistry- Dep., Faculty of Agricultural, Menoufia University
AUTHOR
S. M.
El-Gawad
3
Biochemistry- Dep., Faculty of Agricultural, Menoufia University
AUTHOR
Kh. A.
El-Khalwy
4
Biochemistry- Dep., Faculty of Agricultural, Menoufia University
AUTHOR
ORIGINAL_ARTICLE
BIOCHEMICAL STUDIES ON THE EFFECT OF ONION ON SOME BLOOD PARAMETERS OF RATS
The objective of the present research was to study chemical composition, phenolic compounds and flavonoids of onion. Onion contained total carbohydrates 70.7%, crude protein 7.35 %, total lipids 1.43%, total ash 2.91% fibers 7.91 % and moisture 9.7% based the dry sample. Total phenols in onion bulbs was 35.23 mg /100g., while the total flavonoids was 23.18 mg/100g. HPLC results showed that onion contained 17 phenolic compounds. There were a high percentage of (benzoic acid 905 mg/kg), caticol (649 mg/kg), risvertol (425 mg/kg), perogalol (157 mg/kg) and naringinin (96 mg/kg). that help to improve some blood parameters such as liver function enzymes, kidneys function, lipids and glucose levels. Onion powder as well as ethanolic and water extracts of onion bulbs were prepared which used in the present treatments. Blood parameters of rats were tested using the methods of A.O.A.C.; (2005). Onion powder used as a treatment with concentration of 2 g% and 4 g% of diet. The ethanolic extract of onion used as a treatment with concentration of 1 g/L and 2 g/L. While water extract used as a treatment with concentration of 1 g/L and 2 g/L for hyperlipidemic rats. Blood parameters of rats were tested after the treatments. Data were subjected to statistical analysis using the SPSS (Software version no. 20). Differences between extracts were tested. The best results were obtained by comparisons of the results shown. Treatments with onion powder and its extracts were beneficial to control the hyperlipidemia, and improved the levels of some blood parameters of rats such as, total cholesterol, HDL-c, LDL-c, TG, ALT, AST, creatinine, urea, uric acid, blood glucose.
https://mjab.journals.ekb.eg/article_207625_f06f9671fb5c26989f832ecb527509e8.pdf
2021-11-01
95
105
10.21608/mjab.2021.207625
Onion
HPLC
phenolic compound
Flavonoids
blood parameters
hyperlipidemia
Rats
M. A
Habib
1
Agricultural Biochemistry Department, Faculty of Agriculture, Menoufia University
AUTHOR
Kh. M.
Taha
2
Agricultural Biochemistry Department, Faculty of Agriculture, Menoufia University
AUTHOR
A. F. A.
Sheta
3
Agricultural Biochemistry Department, Faculty of Agriculture, Menoufia University
AUTHOR