ORIGINAL_ARTICLE
EVALUATE THE EFFECT OF YELLOW LUPINE AND FENUGREEK EXTRACTS ON BIOCHEMICAL PARAMETERS ON COTTON LEAF WORM
The Egyptian cotton leafworm is considered the major pest that causes great damage to cotton plants as well as other vegetable crops in Egypt.Great efforts have been made to control this pest chemically. Insecticides of synthetic origin have been used to manage insect pests for more than 50 years. Due to the continuous use of chemical pesticides against this pest, resistance to the action of pesticides had dramatically evolved.
This study was planned to identify the phenolic compounds in ethanol extracts of fenugreek and yellow lupine, on the other hand, investigate the effect of water and alcoholic extracts of fenugreek and yellow lupine on the biochemical responses of the 4th instar larvae ofcotton leafworm. In our study a recommended pesticide namely chlorpyrifos (dursban) was used as a reference. Our results indicated that seven compounds were detected in two ethanolic extracts; gallic acid (164.409 mg /ml) was the major component in fenugreek extract, while catechin was (113.705 mg /ml) the major phenolic compound in yellow lupine extract. All tested extracts showed a significant deterioration in biochemical parameters (GOT, GPT, ALP, ACP activities and total protein levels). The 10 % concentration was the best in both plants extracts, while the ethanolic extract of the fenugreek was the most effective of all the extracts used in the experiment. So, we recommended by using fenugreek and yellow lupine extracts as insecticides to control the cotton leaf worm.
https://mjab.journals.ekb.eg/article_174847_443a1576916cf8e78d51b5e66054c77a.pdf
2019-06-01
37
47
10.21608/mjab.2019.174847
Cotton leafworm
fenugreek
Yellow lupine
Biochemical response
S. N.
Hassab
1
Plant Protection Institute, Agriculture Researche Center, Egypt.
AUTHOR
M. M.
Abozid
2
Agricultural biochemistry department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
S. M.
Abd El-Gwad
3
Agricultural biochemistry department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
A. A.
Ahmed
4
Economic Insectes department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
A. A.
El-Shikh
5
Plant Protection Institute, Agriculture Researche Center, Egypt.
AUTHOR
ORIGINAL_ARTICLE
BIOTECHNOLOGICAL STUDIES ON BIOFUEL PRODUCTION FROM AGRICULTURAL WASTES
Fruits peels, an agricultural waste discarded in huge amounts, were effectively fractionated into its oligosaccharides composition to dedicate their added values utilization. Four fruits peels (Banana, Watermelon, Orange and Mango) were studied. The starch, pectin, hemicellulose, celluloses, lignin and proteins fractions were determined in terms of dry weights percentages for these peels. Mango peels showed the highest oligosaccharides levels, even lignin content was highest by 17.25%. Also, banana peels showed high oligosaccharides levels with the lowest level of lignin by 4.82%. Lab-scale bioreactor was carried out for enzymes production and saccharifaction, the banana and mango peels were released the greatest saccharified pentose and hexose sugars, the total fermentable sugars were 27.77 and 21.13 g.l-1, respectively. Co-fermentation were conducted by selected yeast strain belong to Kluyveromyces marxianus to contribute previouslysexual regenerative Saccharomyces cerevisiae for bioethanol co-production. As expected, co-fermentation increased the productivity by more than 18%, the substantial bioethanol yield were observed by saccharifed banana peels with 10.74 g.l-1, the adding of CaO lead finally to 97.5 wt % of pure bioethanol by duplicate the distillation process.The reaction molar ratio of cocked oil to ethyl acetate were established by 0.1, 0.125, 0.2, 0.25, 0.5 and 1.0 mol.mol-1 respectively. The highest reaction conversion was attained at temperature reaction of 60 °C, molar ratio of oil to ethyl acetate at 1 : 6 ratio (0.17 mol.mol-1) within 6 hours of reaction time, and catalyst concentration of 0.5 wt %.
https://mjab.journals.ekb.eg/article_174852_be7fe83d612fdae355910e0574567160.pdf
2019-06-01
25
35
10.21608/mjab.2019.174852
biofuel
Biodiesel
Agro-peels
fungi co-cultivation
yeast co-fermentation
A.
Elbeltagy
1
Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
Mennah T- Allah
Wagih
2
Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
Wafaa
Hanafy
3
Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
Hanaa
Abo-kora
4
Agric. Microbiol. Res. Dep. Soil, Water and Environment Research Institute, Agricultural Research Center, Egypt.
AUTHOR
S.
Fahim
5
Agricultural Microbiology and Biotechnology, Botany Department, Faculty of Agriculture, Menoufia University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EVALUATION OF SOME PLANT EXTRACTS ON TOXICITY INDUCED BY SOME HEAVY METALS IN EXPERIMENTAL ANIMALS
This study was designed to evaluate the effect of treatment of toxicity and is fully utilized by a variety of raw materials in the body. The results include containing Moringa leaves on a high percentage of protein and using the HPLC device for the plant extract of plant leaves: 23 degrees per minute less than 23 degrees flavonoids, which means that the leaves of the tree Moringa is a good source of flavonoids (antioxidants).
Moral synergies in this study were also supposed to be more effective in this study.
Descriptions of resistance to depression, treatment, treatment and treatment in the body.
It was found that there was an accumulation of cadmium and lead in the internal organs of the mice (liver - kidney) and treatment with extracts of the leaves of Moringa to reduce the acceptable accumulation of these elements in those members.
https://mjab.journals.ekb.eg/article_174855_227adb681d571247d50041d8e8cb44da.pdf
2019-06-01
49
50
10.21608/mjab.2019.174855
Moringa leaves Poisoned by pb
S. N.
Draz
1
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
Samia M.
Khalil
2
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
K. E.
Hussain
3
Lecturer of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
ORIGINAL_ARTICLE
GENETIC IMPROVEMENT OF SOME TRICHODERMA ISOLATES AS BIOLOGICAL CONTROL AGENT
The main objectives of this study were isolation and characterization of some Trichoderma isolates and selection of the best isolates as biocontrol agent. Also improving of these isolates by protoplast fusion and selection of the best strains produced from protoplast fusion. Accordingly, 25 isolates of Trichoderma were obtained and characterized on morphological and molecular bases. It was found that the predominate species in Menoufia governorate was T. harzianum. These isolates showed high ability in inhibition of growth rate of phytopathogenic fungi. Also, they demonstrated different abilities in producing chitinase enzymes (cell wall degradation enzyme for phytopathogenic fungi). Five isolates were selected for improving by protoplast fusion and six isolates obtained from fusion were selected on the basis of their high abilities in hydrolyzing chitin. Also, these strains were better than their parents in inhibition growth rate of phytopathogenic fungi. In addition, they integrated high production of chitinase enzymes. In green house evaluation on faba bean plants, the obtained result showed that the plants treated with these strains and the pathogen were better in growth parameters than the plants treated by the pathogen only. Also, this treatment showed better growth parameters than the plants treated by parenteral isolates and the phytopathogen.
https://mjab.journals.ekb.eg/article_174860_393f0df859f1d61c7c016e0ee7223c53.pdf
2019-06-01
51
52
10.21608/mjab.2019.174860
morphological features
ITS and TEF1-α
Mycoparasitism
Chitinase
Exochitinase, Genetic Improvement, Protoplast Fusion
Ragaa A.
Eissa
1
Prof. of Genetics, Fac. of Agric., Menoufia Univ.
AUTHOR
A. I.
Fahmi
2
Prof. of Genetics, Fac. of Agric., Menoufia Univ.
AUTHOR
A. M.
El-Zanaty
3
Assistant prof. Genetics, Fac. of Agric., Menoufia Univ.
AUTHOR