ORIGINAL_ARTICLE
COMPARATIVE STUDY ON LUPINE AND FENUGREEK SEEDS THAT GROW IN EGYPT
Main chemical composition, bioactive constituents and antioxidant activity of Egyptian lupine and fenugreek seeds were investigated. The obtained results showed that white lupine seeds have higher amount of carbohydrate and ash than that in the other seeds. Meanwhile yellow lupine seeds showed the highest percentage of fibers among all tested seeds, while fenugreek seeds were characterized by large amount of crude protein, oil and moisture comparing with both kinds of lupine seeds.
On the other side, analysis of bioactive constituents revealed the presence of total phenolics, total flavonoids as well as saponins in the highest amount in fenugreek seeds, whereas the highest values of alkaloids and tannins were belonged to yellow lupine. It is noteworthy that white lupine exhibited the lowest amounts of all tested bioactive components among all studied seeds.
And finally, in vitro antioxidant activity using two deferent methods, showed a correlation between total phenolics and total flavonoids on one hand and antioxidant activity on the other hand, where it showed the highest values for fenugreek seeds followed by yellow and white lupine seeds respectively.
https://mjab.journals.ekb.eg/article_169568_5d146ab3323063200de1a5a232078080.pdf
2020-02-01
23
33
10.21608/mjab.2020.169568
lupines (white and yellow) and fenugreek seeds
alloxan
diabetic rats
Hypoglycemia
Antioxidant enzymes
S.A.
Elkadousy
1
Biochemistry department, Faculty of Agriculture, Menoufia University
AUTHOR
Samia. M.
Khalil
2
Biochemistry department, Faculty of Agriculture, Menoufia University
AUTHOR
Houda A.
Fareed
3
Biochemistry department, Faculty of Agriculture, Menoufia University
AUTHOR
H. M.
Mahmoud
4
Management of snails control in Giza
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF GINGER AND GINSENG RHIZOMES ON LIVER FUNCTION IN HYPERLIPIDEMIC RATS
The study aimed to investigate the effect of ginger and ginseng rhizome on liver functions in hyperlipidemic rats. The addition of 5% or 10% ginger and ginseng to the feed of hyperlipidemic rats produced decreased total cholesterol level, Triglycerides as well as HDL-c, LDL-c and VLDL-c levels.
The liver enzymes AST, ALT and ALP showed an increase in their activities in the rats feed on hyperlipidemic diet comparing with normal control. Using ginger and ginseng decreased the activities of these enzymes compared to the positive control. AST/ALT ratio recorded values under 5 (between 1.02 and 1.07) this proved that the harmful of induced hyperlipidemic effect liver. Hyperlipidemic diet produced a decreased in total protein and albumin in blood. The addition of ginger and ginseng increased the amount of total protein and albumin compared to positive control.
https://mjab.journals.ekb.eg/article_169569_5e5c872a03fb33563a2e35d8030223fe.pdf
2020-02-01
1
10
10.21608/mjab.2020.169569
Lipid
Liver
kidney
antioxidant
hyperlipidemic
Cholesterol
Phenolic
Enzyme
rhizome
M.A.
Hbib
1
Department of Biochemistry Faculty of Agric. Fac. Menoufia. Unv.
AUTHOR
A.M.
Farid
2
Department of Biochemistry Faculty of Agric. Fac. Menoufia. Unv.
AUTHOR
H.El.A.
Farid
3
Department of Biochemistry Faculty of Agric. Fac. Menoufia. Unv.
AUTHOR
S.H.M.
Hebisha
4
Department of Biochemistry Faculty of Agric. Fac. Menoufia. Unv.
AUTHOR
ORIGINAL_ARTICLE
HEPATOPROTECTIVE EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIS FROM CYMBOPOGAN CITRATUS (LEMONGRASS)
Green synthesis of silver nanoparticles (AgNPs) is non-toxic, rabid and eco-friendly than commonly used physicochemical methods. This study focuses on synthesis, characterization, hepatoprotective effect and antioxidant activity of AgNPs synthesizes from Cymbopogan Citratus (Lemongrass). Silver nanoparticles were formed within 30 minutes by heating to 80oC magnetic stirrer using aqueous solution of AgNO3 (0.1 N) with fresh leaves methanolic extract of Cymbopogan Citratus. The synthesized silver nanoparticles were characterized by using UV-visible spectrophotometer analysis and transmission electron microscope (TEM). The antioxidant activity of nanoparticles were determined in vitro by reducing power, total antioxidant capacity and free radical scavenging activity by (DPPH). and experimental study was designed to evaluate the antioxidant activity and hepatoprotective effect of silver nanoparticeles, methanolic extract and essential oil comparing with vitamin E.
Results: (UV-Vis) spectrum of synthesized AgNPs shows a peak at 412 nm and the size of silver nanaoparticels was 13.5 nm by (TEM).
Silver nanoparticles showed potent hepatoprotective effect and antioxidant activity in both in vitro and in vivo methods.
https://mjab.journals.ekb.eg/article_169570_a5b19812c7a56f4e7e8f9720de6ddcaf.pdf
2020-02-01
11
22
10.21608/mjab.2020.169570
Silver nanoparticles (AgNPs)
Green synthesis
Lemon grass
Antioxidant activity
DPPH (2
2-diphenyl -1- pricrylhydrazyl)
M. A.
Hammam
1
Department of Biochemistry Faculty of Agricultural Menofia Univeristy shibin El-Kom, Egypt
AUTHOR
G. A.
Khalil
2
Department of Biochemistry Faculty of Agricultural Menofia Univeristy shibin El-Kom, Egypt
AUTHOR
S. M. Abd
ElGawad
3
Department of Biochemistry Faculty of Agricultural Menofia Univeristy shibin El-Kom, Egypt
AUTHOR
Manal K. I. El-
Bisi
4
Prof. Doctor at chemical and technology of textile National research center
AUTHOR
I. A. A.
Mohamed
5
Ministry of Health and Population, Egypt.
AUTHOR
ORIGINAL_ARTICLE
BIOCHEMICAL STUDIES ON SOME NANOTECHNOLOGY APPLICATIONS
Recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. our study aims to introduce a simple, rapid and economical route to synthesize silver nanoparticles (AgNPs) using aqueous and methanolic extracts from two medicinal plants; ginger and lemon grass as reducing and stabilizing agent, AgNPs synthesis was inferred by the change of color from yellow to brown. The size, morphology and stability of AgNPs depend on the active ingredients in the extracts. AgNPs size and morphology were characterized using Ultraviolet-visible (UV-Vis) spectrum of synthesized AgNPs shows a peak from 410-425 nm approximately. and transmission electron microscopy (TEM) analysis indicated spherical shape of nanoparticles with average size of 13.0 - 35 nm. We investigate the antioxidant effect of (AgNPs) synthesized from aqueous and methanolic extracts in both in vitro and in vivo, and we can conclude that, silver nanoparticles recorded the most potent in vitro and in vivo antioxidant effect and can protect liver, kidney and heart of rats against oxidative stress caused by H2O2 (0.5 % in drinking water), also have antibacterial effect against some human pathogenic bacteria.
https://mjab.journals.ekb.eg/article_169571_065ba2c5a4c1cffa71ac18b4bc2ffaa3.pdf
2020-02-01
41
42
10.21608/mjab.2020.169571
Silver nanoparticles (AgNPs)
Green synthesis
Ginger
Lemon grass
Antioxidant activity
M. A.
Hammam
1
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
S. M. Abd
ElGawad
2
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
Manal K. I. El-
Bisi
3
Prof. of Textile Chemistry and Technology, National Research Center, Dokki, Cairo.
AUTHOR
G. A.
Khalil
4
Associate prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
ORIGINAL_ARTICLE
THE HYPOGLYCEMIC EFFECT OF SOME COMMON PLANTS THAT GROW IN EGYPT
The study aimed to study, the chemical composition of lupine (bitter and sweet) seeds and fenugreek seeds and their content of active chemicals that have a hypoglycemic effect and also the effect of lupine (bitter and sweet) seeds powdere and fenugreek seeds powder and the combination of bitter lupine (10%) and fenugreek (10%) on the increase of glucose level, enzymes, indications (liver, kidneys, lipid and antioxidants activity) and liver tissues in diabetic rats induced-alloxan.
The addition of 20% of lupine (bitter and sweet) seeds powder, fenugreek seeds powder, and the combination of bitter lupine (10%) and fenugreek (10%) with a diet meal for diabetic rats led to decrease of glucose level, enzymes and indications of (liver, kidneys, total colesterole, triglyceride levels, LDL-c and antioxidants activity) also led to an increase in HDL-c, in diabetic rats compared with diabetic control.
The addition of 20% lupine (bitter and sweet) seeds powder and fenugreek seeds powder and the combination of bitter lupine seeds (10%) and fenugreek seeds (10%) improved oxidative stress and kept liver cells from damage compared to diabetic control
https://mjab.journals.ekb.eg/article_169572_d9436fcbcf664754fbecc7628fed1155.pdf
2020-02-01
39
40
10.21608/mjab.2020.169572
lupines (white and yellow) and fenugreek seeds
alloxan
diabetic rats
Hypoglycemia
Antioxidant enzymes
S. A. El
kadousy
1
Prof. of Agric. Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
Samia M.
Khalil
2
Prof. of Agric. Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
Houda A.
Fareed
3
Prof. of Agric. Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
ORIGINAL_ARTICLE
BIOCHEMICAL STUDIES ON SOME PLANT EXTRACTS.
The present work aims to study the potential effect of jamun Syzygium cumini seeds and leaves to give more protection against hyperlipidemia . Twenty male albino rats were used over 30 days period. The animals were divided into (4) groups, wherein groups number (1) represent control which were fed basal diet, while group number (2) was received high fat diet to serve as hyperlipidemic group. Other two groups allowed to feed high fat diet supplemented with jamun seeds and leaves, group number (3) treated simultaneously with jamun seeds, fourth group treated with jamun leaves. At the end of the experimental period, blood samples were collected to determine lipid profile , liver functions From the obtained results we concluded that group of rats fed on high fat diet were considered as a major risk factor for hyperlipidemia disease. Our results could be summarized that jamun seeds and jamun leaves were considered the best for causing a reduction of TC, TG and LDL. Also, liver functions has been improved and there were significant reduction GOT, GPT and ALP activity than that of positive control group. It could be concluded that jamun seeds and jamun leaves under study are useful for the treatment of hyperlipidemia.
https://mjab.journals.ekb.eg/article_169573_3c26053ae299ccb5bafba1b78deb2552.pdf
2020-02-01
35
36
10.21608/mjab.2020.169573
Jamu
Liver
Kideny Antioxidant
Hiperlipidemic
Cholesterol
Phenolic
Enzymes
seeds
leaves
M. A.
Hamma
1
Prof. of Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
S. A. El-
Kadousy
2
Prof. of Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
S. M. Abd El-
Gawad
3
Prof. of Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
ORIGINAL_ARTICLE
BIOCHEMICAL STUDIES ON SOME MEDICINAL PLANTS
This study was carried out to evaluate the effect of ginger and ginseng roots in diets on lipid fraction of hyperlipidemic male albino rats . the investigation was done with diets contain 5% or 10% roots of ginger and ginseng to evaluate their effects as hyperlipidemic agents which amounted the above concentration of the diets at the expense of starch. The analysis of the tow roots presence of 17 phenolic compounds in ginger, while ginseng recorded 20 phenolic compounds, Varying in their levels, The obtained results showed that the roots of ginger and ginseng generally improved the clinical blood status (RBCs, WBCs, Hb, Plat), lipid fraction, total cholesterol level, Triglycerides as well as HDL-c, LDL-c and VLDL-c, also the treatment alleviated the risk factor of the hyperlipidemic rats. Liver function (AST, ALT, ALP and GGT) activity as well as total and direct bilirubins. Kidneys function (Urea, Creatinin and Uric acid), Total protein and its fractions (Albumin and globulin), lipids peroxidation and antioxidative enzymes activity (SOD, CAT, and MDA) were readjusted around the improvements of the present lipotropic factors and antioxidative agents of ginger and ginseng roots which hyperlipidemia were alleviated. 10% ginger or 10% ginseng showed the best results compared with the another treatments
https://mjab.journals.ekb.eg/article_169574_6354455abb8e5769299bfbb5647911a1.pdf
2020-02-01
37
38
10.21608/mjab.2020.169574
Biochemical studies
medicinal plants ginger
albino rats
Lipid fraction
M. A.
Habib
1
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
A. M.
Farid
2
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR
Hoda E. A.
Farid
3
Prof. of Agric Biochemistry, Fac. of Agric., Menoufia Univ.
AUTHOR