@article { author = {Mandour, A. and Fahim, S. and Hussein, Walaa and Hanafy, Wafaa and Elbeltagy, A.}, title = {IMPACT OF ENDOPHYTIC LIPOPEPTIDES USE AGAINST SOME PLANT PATHOGENIC MICROORGANISMS}, journal = {Menoufia Journal of Agricultural Biotechnology}, volume = {5}, number = {2}, pages = {43-65}, year = {2020}, publisher = {Menoufia University, Faculty of Agriculture}, issn = {2357-0768}, eissn = {2735-3532}, doi = {10.21608/mjab.2020.170407}, abstract = {Twelve dissimilar plant associated bacterial colonies were isolated from five different tomato seedlings, the Non Ribosomal Lipopeptides genes were detected by degenerated primers technique in the twelve tomato endophytic isolates, only the long rode bacilli endophytic strains were found to harbour the lipopeptides synthetases genes, these three strains were given laboratory names of (BMG100, BMG101 and BMG102) for further pursuit. Partial sequencing of 16s RNA gene and protein profile analysis for the three bacilli isolates showed that all isolates belonged to Bacillus subtilis. The lipopeptides production by HPLC analysis revealed that all the endophytic isolates from tomato stems had the ability to produce lipopeptides except the third isolate named B. subtilis BMG102 showed no production. different levels of volumetric oxygen transfer coefficient (KLa) were used as cursors for lipopeptides production. The maximum concentration of surfactin type was about 1431, 529 and 437 mg.L-1, the plipastitin (fengycin type )  was 360 198 mg.L-1  and zero while the  Mycosubtilin (iturin type) was zero, 228 and 414 mg.L-1 for the strains B. subtilis ATCC21332, BMG100 and BMG101, respectively. Surfactin production is visibly optimal at high oxygen transfer (kLa) and showed more than 0.07 S-1, while the optimum production of the fengycin and iturin could be observed at mild values of O2 transfer (kLa) which equal 0.01 and 0.015 S-1 respectively. The antibacterial - antifungal effects of the three B.subtilis extracts collected during  lipopeptides productivity regimes showed inhibition activity against Pseudomonas syringae, Ralstonia solanacearum and Erwinia amylovora, as will as the antifungal activity against Aspergillus niger, Phanerochaete chrysosporium and Alternaria alternata. These results indicate potential use of B.subtilis strains as biocontrol agents, especially when considering commercial implementation. Studies regarding the lipopeptides applications, should also be considered as this will influence the efficacy of the lipopeptides against the target organisms.}, keywords = {Endophytic,Lipopeptides,plant Pathogens,biocontrol agents}, url = {https://mjab.journals.ekb.eg/article_170407.html}, eprint = {https://mjab.journals.ekb.eg/article_170407_98d4b7a112b70ce05f4d06013d263a30.pdf} } @article { author = {Fekry, A. and Fahim, S. and Hussein, Walaa and Hanafy, Wafaa and Elbeltagy, A.}, title = {INVESTIGATION OF VIRULENT AND TOXIN GENES EXPRESSION IN SOME SALMONELLA SEROVARS}, journal = {Menoufia Journal of Agricultural Biotechnology}, volume = {5}, number = {2}, pages = {67-81}, year = {2020}, publisher = {Menoufia University, Faculty of Agriculture}, issn = {2357-0768}, eissn = {2735-3532}, doi = {10.21608/mjab.2020.170411}, abstract = {Samples collected from patient stool during their course of investigation at hospitals ministry of health, Egypt as well as solid and liquid food samples collected from local markets were examined for presence of Salmonella spp. Isolation and serological identification showed that 92.5% of collected samples contained Salmonella spp belonged to six serovars; Salmonella   , enteritidis, Newport, derby, senftenberg and Virchow. These six serovars were checked by PCR for presence of three genes involved in virulence and toxin production, invA, avrA and stn. The gene invA was detected in all serovars, while avrA and stn genes were found in only 4 serovars. In this regard, S.typhimurium    was found to harbour the three tested genes. When Salmonella  typhimurium  grew on normal synthetic medium and natural animal cells (Red blood; RBC and murine epithelial cells; IEC-6) and incubated separately at different temperatures 5, 25, 35 and 40 C for 60 h., they showed regular increase in growth rate with increasing temperature. Also, total RNA concentrations increased up to 24 h. then slightly decreased at each examined temperature specially at 25, 35 and 40 C, but the highest RNA concentration was observed at 40 C. indicating its preparedness in the form of metabolic and cellular activities to cope with temperature stress. Moreover, S. typhimurium seems to be more potent and showed increased ability to induce the expression of enterotoxin gene (stn) by the exposure of temperature elevation specially when grown on murine epithelial cells; IEC-6 comparing with synthetic media or red blood cells.}, keywords = {Salmonella,Virulent genes,invA gene,avrA gene,enterotoxin gene (stn)}, url = {https://mjab.journals.ekb.eg/article_170411.html}, eprint = {https://mjab.journals.ekb.eg/article_170411_82450d2de4970fe857fd3a9e1df2e924.pdf} } @article { author = {Ashoush, Y. and Abozid, M. and Mansour, S. and Morgan, A.}, title = {EFFECT OF CHLORPYRIFOS ON LIVER FUNCTION OF ALBINO RATES}, journal = {Menoufia Journal of Agricultural Biotechnology}, volume = {5}, number = {2}, pages = {83-92}, year = {2020}, publisher = {Menoufia University, Faculty of Agriculture}, issn = {2357-0768}, eissn = {2735-3532}, doi = {10.21608/mjab.2020.170415}, abstract = {This study was conducted with the aim of studying the survival of chlorpyrifos on cucumber fruits. The fruits were applied by the recommended doses of chlorpyrifos. Residues of pesticides in fruits were determined used liquid chromatography. The results showed that the residual chlorpyrifos decreased gradually due to time factor and the process of the fruit washing with water had a clear effect in reducing the concentration of the presence of chlorpyrifos in the applied fruits. Chronic toxicity of chlorpyrifos on liver function after the pesticide application showed that pesticides induced some effects on different biological functions of experimental animals applied with pesticide.}, keywords = {chlorpyrifos,liver function,Cucumber,Pesticides residues}, url = {https://mjab.journals.ekb.eg/article_170415.html}, eprint = {https://mjab.journals.ekb.eg/article_170415_5970a1c7de863a47dbdd4d0f954f0582.pdf} } @article { author = {shuony, F. M. El- and Kadousy, S. A. El- and ziead, M. M. Abo}, title = {THE PROTECTIVE EFFECTS OF SOME PLANTS ON RATS TREATED WITH HEAVY METALS}, journal = {Menoufia Journal of Agricultural Biotechnology}, volume = {5}, number = {2}, pages = {93-94}, year = {2020}, publisher = {Menoufia University, Faculty of Agriculture}, issn = {2357-0768}, eissn = {2735-3532}, doi = {10.21608/mjab.2020.170419}, abstract = {A water and ethanolic extracts for both plants (ginger and turmeric) were determined, from point of view of their content of total phenolics and flavonoids their antioxidant activity using DPPH method , total antioxidant capacity and reducing power assay and the HPLC analysis of ethanolic extract was performed to determine active compounds. The mice were treated with a 1/2 LD50 of the heavy metals ( lead - nickel ) together with different plant extracts ( ginger - curcuma ) to determine the influence of such compounds in vivo experiment  by measuring of some biomarkers to determine the recovery extent of liver, kidneys, heart and total lipid profile as well as antioxidant biomarkers. It was found that there was a significant improvement in the levels of such biomarkers at all. Where the levels of antioxidant biomarkers, showed an increased in general in SOD, GPx , GR, GST, G6PDH, CAT, GSH and a decreased in MDA levels; from the level of liver function markers, kidneys function, total lipid profile markers and heart functions was observed also, all treatments  compared to the control groups. The ginger extracts were more effective than curcuma ones comparing with positive control, and that the ethanol extracts were more effective them that of aqueous extracts as antioxidant and as protective agent against cells damage.}, keywords = {Ginger,Turmeric,antioxidant,Oxidative Stress}, url = {https://mjab.journals.ekb.eg/article_170419.html}, eprint = {https://mjab.journals.ekb.eg/article_170419_23a413883a20e7fc49fbc3ad6ae3674c.pdf} }